男女18禁啪啪无遮挡激烈网站,鸭子TV国产在线永久播放,最近中文字幕在线MV视频在线,四川妇女BBBB的创业精神

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>FL62891人肝細(xì)胞

人肝細(xì)胞

簡要描述:CRL-11005 FL62891 人肝細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;
細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,
提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:FL62891
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-12
  • 訪  問  量:2086

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-11005 FL62891 人肝細(xì)胞

Designations: FL 62891
Depositors: ImClone Systems Inc.
Biosafety Level: 2 [Cells contain SV-40 viral DNA sequences ]
Shipped: frozen


Medium & Serum: San)ee PropagationATCC® Number: CRL-11005™ Price: $353.00


Growth Properties: adherent

Organism: Homo sapiens (hum

Morphology: fibroblast

CRL-11005 FL62891 人肝細(xì)胞

Source: Organ: liver

Cell Type: immortalized with SV40 large T antigen

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Age: fetus

Comments: The cell line was derived from human fetal liver tissue, and immortalized by transformation with an ecotropic retrovirus containing a temperature sensitive SV40 T-antigen (tsA58) and a G418 resistance gene. After several days at 37C, the temperature of the medium was lowered to 32C. Cells were selected with G418. The selected cells were expanded and maintained. The cells produce flk-1 and some flk-2 ligands.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, and 15% fetal bovine serum

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 32.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 32°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 32°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 48 hours

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020

References: 70742: Lemischka IR. Nucleic acids encoding fragments of hematopoietic stem cell receptor flk-2. US Patent 5,270,458 dated Dec 14 1993

70744: Lemischka IR. Tyrosine kinase receptor flk-2 and fragments thereof. US Patent 5,367,057 dated Nov 22 1994

70745: Lemischka IR. Tyrosine kinase receptor human flk-2-specific antibodies. US Patent 5,548,065 dated Aug 20 1996

70746: Lemischka IR. Nucleic acids encoding soluble human FLK-2 extracellular domain. US Patent 5,621,090 dated Apr 15 1997

70747: Lemischka IR. Antibodies against tyrosine kinase receptor flk-1. US Patent 5,747,651 dated May 5 1998

70748: Lemischka IR. Method for isolating stem cells expressing flk-1 receptors. US Patent 5,912,133 dated Jun 15 1999



CRL-11005 FL62891



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
男女18禁啪啪无遮挡激烈网站,鸭子TV国产在线永久播放,最近中文字幕在线MV视频在线,四川妇女BBBB的创业精神

                  国产精品久久久久久| 国产女人18毛片视频| 国产成人精品国内自产拍观看| 欧美日韩一区二区三区| 亚洲激情区| 天堂中文在线资源| 欧美人xxxx| 大香蕉最新在线视频| 97在线播放视频| 半夜摸妺妺乳C起来好疼| 日本老熟妇乱| 久久国产成人午夜av影院| 女人18岁毛片| 国产精品国产三级国产AV主播| 人人爽人人做人人揉人妻| 国产精品一区二区三区高潮| 欧洲尺码日本尺码美国欧洲LV| 亚洲欧美综合区丁香五月小说| 韩国三级大胸波霸| 中文字幕人妻熟妇又伦精品免bl| 丁香花在线观看免费高清完整版 | 久久久久久久久久久久亚洲| japanesexxxx日本熟妇伦视频| 丰满少妇被爽到高潮喷水| 欧美丰满少妇| 高清人妻| 少妇精油按摩高潮| 公交车大龟廷进我身体里视频| 欧美黑人乱大交| 亚洲精品成人天堂一二三| 国产内射精品| 国产福利一区二区三区在线观看视频| 成人性生活大片免费看r| 久久人人爽爽爽人久久久| silk一区二区三区精品视频| 99久久久国产精品| 99热最新在线| jux875人妻本庄优花| 草草影院ccyy国产欧美| 精品国产免费一区二区三区五区| 国产精品午夜视频|